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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461337
VAT: CZ60461373
Czech Post certified digital mail code: sp4j9ch
Copyright UCT Prague 2017
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre
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[obsah] => Study programmes provided at the Faculty of Food and Biochemical Technology (FFBT) embrace both traditional fields of food science as well as current trends in the development of food and biochemical sciences. Traditional fields such as brewing, sugar manufacture, fermentation, and agrochemistry were taught at UCT Prague's historical predecessor, Prague Polytechnic, as early as the beginning of the last century.
Since the formation of the independent Institute of Chemical Technology, Prague (now University of Chemistry and Technology, Prague) in 1952, the Faculty has grown into an institution with a broad background in chemical, biochemical, engineering, and other disciplines, which illustrates the purposefulness of such a strong chemical-technological complex and the FFBT as its inseparable part—even on the international scale. The Faculty ranks among leading university centres of food science. In the course of its existence, the Faculty has turned out thousands of graduates who pursue their careers primarily in the food industry and related fields, in scientific research, and at schools and institutions of higher learning both in the Czech Republic and abroad.
The concentration of university teaching in all food-related fields—including applied biology and biochemistry—at FFBT represents one of the major strengths of the Faculty's existence within UCT Prague. However, besides advocates of the Faculty's existence within a chemistry university, naturally there are also many critics of such integration. Their major argument is that the food industry (the meat, dairy, and preservation industries in particular) as well as other fields are closely interrelated with farming production and should be taught at institutions focused on agriculture or veterinary science. Many agricultural and other universities and institutions of higher learning in the Czech Republic have lately introduced selected fields of food science in their study programmes, which will undoubtedly enhance desirable competition between their graduates.
The extent of this introduction to FFBT does not allow for a detailed description of the numerous diverse scientific projects implemented by the Faculty at present. We can only provide basic information to our future students and partner organizations about the scientific and research orientations of individual departments at the Faculty. All departments offer cooperation in scientific research and development; expertise and consulting; and continuing education for specialists and scientific workers in the form of various postgraduate programmes, short-term courses, and long-term scholarships. The Faculty's academic staff also prepare short-term specialized and retraining courses tailored to the needs of individual clients. Individual departments offer basic and specific analyses based on the most sophisticated methods and using state-of-the-art analytical apparatuses. The extent of consulting, expertise, and research and development activities is not restricted to the areas listed above, but it may be further expanded according to specific needs of industrial practice.
I believe that this introduction will provide basic information about the orientation of the Faculty and various forms of study offered by its individual departments, and that it will help us find new partners for cooperation in solving research and professional problems.
prof. Ing. Karel Melzoch, CSc
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- Department of Biotechnology
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- Department of Saccharides and Cereals
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- Department of Food Analysis and Nutrition
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Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
Cryo-EM structural biology of diseases: Rationally designed viral-RNA based inhibitors of influenza RNA polymerase
| Granting Departments: |
Department of Biochemistry and Microbiology
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | Mgr. Tomáš Kouba, Ph.D. |
Annotation
Effective treatment against influenza viruses (Flu) suffers from virus resistance. Therefore, there is a great demand for exploring new drug targets and developing effective novel antivirals. This is challenging, since there is only a limited number of antiviral targets for rational drug design. This project will tackle both; it will explore and verify a new target and develop inhibitors against it. Cutting edge results in structural biology of the influenza RNA-dependent RNA polymerase (FluPol), identified a common general mechanism of Flu RNA transcription and replication cycle. The mechanism is entirely dependent on binding of viral endogenous RNA molecules to specific sites on the FluPol. Aided by already existing cryo-EM structures of FluPol, we will design inhibitors based on the sequence, atomic structure and interactions with the protein, and add extensive chemical modification to these viral RNAs. We will then test whether they can inhibit FluPol by in vitro and in cell-based experiments. Targeting these RNA binding sites and using RNA-scaffold based molecules is an innovative concept and expected to be robust in respect to the development of viral resistance. Altogether, the approach has great potential to bring a new universal paradigm to the field of antiviral drug discovery.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
Exploring Respiratory Viral Entry: Comparative Insights from the Interactomes of Coronaviruses and Influenza A Viruses during Early Infection
| Granting Departments: |
Department of Biochemistry and Microbiology
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Microbiology ( in English language ) |
| Supervisor: | Mgr. Jan Weber, CSc. |
Annotation
The entry of viruses into host cells is a crucial initial step, particularly for respiratory viruses with significant pandemic potential such as SARS-CoV-2 and influenza A virus. Analyzing the interactomes of coronaviruses and influenza A virus and comprehending the early interactions between viral and cellular proteins can provide valuable insights for developing targeted or broad-spectrum antiviral therapies. The main goal of this project is to understand the viral and cellular factors influencing the entry of respiratory viruses. We will employ several strains of coronaviruses and influenza A virus, known to exploit different entry routes into the host cell, and will compare their cellular interactome at the time of entry and during early post-entry events. From the identified cellular interacting partners, interesting candidates will be selected and their role in the virus life cycle analyzed. The student will learn how to work with cell cultures in biosafety level 3, perform proteomic and bioinformatic analyses, and conduct siRNA experiments for gene-knockdown in mammalian cells infected with various viruses. The proposed project will broaden our current knowledge about the early phase of infections of mammalian cells with respiratory viruses.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
Modified nucleotides for selection of functional nucleic acids and labelled probes for chemical biology
| Granting Departments: |
Department of Chemistry of Natural Compounds
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | prof. Ing. Michal Hocek, DSc. |
Annotation
We will design and synthesize modified nucleoside triphosphates bearing diverse functional groups for enzymatic synthesis of modified oligonucleotides which will be applied in selection and construction of new functional nucleic acids, e.g. aptamers or aptazymes and for the construction of fluorescent or redox probes for applications in chemical biology. References: 1. Hocek, M.: "Enzymatic Synthesis of Base-Functionalized Nucleic Acids for Sensing, Cross-linking, and Modulation of Protein–DNA Binding and Transcription" Acc. Chem. Res. 2019, 52, 1730-1737. 2. Micura, R.; Höbartner, C. Fundamental studies of functional nucleic acids: aptamers, riboswitches, ribozymes and DNAzymes. Chem. Soc. Rev. 2020, 49, 7331–7353.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
New cytostatic nucleosides and nucleotides with new mechanisms of action
| Granting Departments: |
Department of Chemistry of Natural Compounds
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | prof. Ing. Michal Hocek, DSc. |
Annotation
We will design and synthesize new modified nucleosides and nucleotides as potential cytostatic agents with new mechanisms of action which includes modulation of receptors or activation of cytostatic proteins. Selected active compounds will be further optimized in order to identify preclinical drug candidates. References: 1. Jordheim, L. P.; Durantel, D.; Zoulim, F.; Dumontet, C. Advances in the development of nucleoside and nucleotide analogues for cancer and viral diseases. Nat. Rev. Drug Discov. 2013, 12, 447–464. 2. Perlíková, P.; Hocek, M. Pyrrolo[2,3-d]pyrimidine (7-deazapurine) as a privileged scaffold in design of antitumor and antiviral nucleosides. Med. Res. Rev. 2017, 37, 1429–1460.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
Preparation and applications of charged heterodienes in bioorthogonal reactions
| Granting Departments: |
Department of Chemistry of Natural Compounds
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | Ing. Milan Vrábel, Ph.D. |
Annotation
Our group recently described the development and first applications of N1-alkyl-1,2,4-triazinium salts in bioorthogonal reactions (Angew. Chem. Int. Ed., 2023, e202306828). In this project, we want to explore the chemistry of these charged heterodienes in more detail. In addition, we want to apply the developed reagents in applications ranging from selective modification of biomolecules to cellular applications (e.g. bioimaging). The project combines synthetic organic chemistry, reaction kinetics and stability studies with biological experiments that will be performed mainly in collaboration with biologists in the group.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
Regulation of growth and metabolism
| Granting Departments: |
Department of Biochemistry and Microbiology
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | David Marcelo Sabatini, M.D., Ph.D. |
Annotation
We have a long-standing interest in the regulation of growth and metabolism that stems from our early work on the pathway anchored by mTOR protein kinase. We now appreciate that it is a major regulator of growth and anabolism in animals and responds to diverse stimuli, including nutrients. Because our work revealed that lysosomes play a key role in the activation of mTORC1 by nutrients, we began to study lysosomes as well as other organelles. Available thesis projects: (1) Nutrient sensing by mTOR. We seek to: discover the glucose sensor for mTORC1 and nutrient sensors in animals beyond mammals; understand how the known nutrient sensors function in vivo; and elucidate the biochemical function of GATOR2. (2) Lysosomes in normal physiology and disease. Using the Lyso-IP methodology and CRISPR screening we seek to understand how neurodegenerative diseases impact lysosomes and identify the contents of lysosomes in specialized cells, like immune cells. (3) Methods to study small molecule metabolism in vivo. We seek to develop methods to study carbohydrate metabolism in cells in vivo in mice. (4) Development of drug-like molecules. In collaboration with chemists at IOCB and elsewhere, we seek to develop drug-like molecules that target mTOR pathway components as well lysosomal and mitochondrial proteins.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
Rhomboid protease inhibitors
| Granting Departments: |
Department of Biochemistry and Microbiology
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | Ing. Kvido Stříšovský, Ph.D. |
Annotation
The proposed PhD project will be focused on the development of inhibitors and probes for rhomboid intramembrane proteases, which have been implicated in the regulation of mitophagy, malaria parasite invasion and epithelial homeostasis. Prior work in the thesis advisor’s lab has shown that peptidyl ketoamides are potent rhomboid protease inhibitors. The aim of the thesis will be to establish platforms to investigate and improve the potency and selectivity of this class of compounds for rhomboids and develop potent inhibitors and probes for specific rhomboid proteases implicated in Parkinson’s disease and epithelial homeostasis. These compounds will enable and facilitate biological studies of rhomboid proteins and serve as starting points for potential pharmacological applications.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
Sequence-specific enzymatic synthesis of base-modified RNA
| Granting Departments: |
Department of Chemistry of Natural Compounds
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i. |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | prof. Ing. Michal Hocek, DSc. |
Annotation
We will design and synthesize modified ribonucleoside triphosphates bearing diverse functional groups at nucleobase. These nucleotides will be used for sequence-specific enzymatic synthesis of oligoribonucleotides (RNA) bearing labels or modifications at specific positions using engineered DNA polymerases. The applications will include tRNA, mRNA, sgRNA etc. References: 1. Micura, R.; Höbartner, C. Fundamental studies of functional nucleic acids: aptamers, riboswitches, ribozymes and DNAzymes. Chem. Soc. Rev. 2020, 49, 7331–7353. 2. Milisavljevic, N.; Perlíková, P.; Pohl, R.; Hocek, M. Enzymatic synthesis of base-modified RNA by T7 RNA polymerase. A systematic study and comparison of 5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside triphosphates as substrates. Org. Biomol. Chem. 2018, 16, 5800-5807.
Contact supervisor
Study place:
Institute of Organic Chemistry and Biochemistry of the CAS, v. v. i.
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