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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461337
VAT: CZ60461373
Czech Post certified digital mail code: sp4j9ch
Copyright UCT Prague 2017
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre
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[obsah] => Study programmes provided at the Faculty of Food and Biochemical Technology (FFBT) embrace both traditional fields of food science as well as current trends in the development of food and biochemical sciences. Traditional fields such as brewing, sugar manufacture, fermentation, and agrochemistry were taught at UCT Prague's historical predecessor, Prague Polytechnic, as early as the beginning of the last century.
Since the formation of the independent Institute of Chemical Technology, Prague (now University of Chemistry and Technology, Prague) in 1952, the Faculty has grown into an institution with a broad background in chemical, biochemical, engineering, and other disciplines, which illustrates the purposefulness of such a strong chemical-technological complex and the FFBT as its inseparable part—even on the international scale. The Faculty ranks among leading university centres of food science. In the course of its existence, the Faculty has turned out thousands of graduates who pursue their careers primarily in the food industry and related fields, in scientific research, and at schools and institutions of higher learning both in the Czech Republic and abroad.
The concentration of university teaching in all food-related fields—including applied biology and biochemistry—at FFBT represents one of the major strengths of the Faculty's existence within UCT Prague. However, besides advocates of the Faculty's existence within a chemistry university, naturally there are also many critics of such integration. Their major argument is that the food industry (the meat, dairy, and preservation industries in particular) as well as other fields are closely interrelated with farming production and should be taught at institutions focused on agriculture or veterinary science. Many agricultural and other universities and institutions of higher learning in the Czech Republic have lately introduced selected fields of food science in their study programmes, which will undoubtedly enhance desirable competition between their graduates.
The extent of this introduction to FFBT does not allow for a detailed description of the numerous diverse scientific projects implemented by the Faculty at present. We can only provide basic information to our future students and partner organizations about the scientific and research orientations of individual departments at the Faculty. All departments offer cooperation in scientific research and development; expertise and consulting; and continuing education for specialists and scientific workers in the form of various postgraduate programmes, short-term courses, and long-term scholarships. The Faculty's academic staff also prepare short-term specialized and retraining courses tailored to the needs of individual clients. Individual departments offer basic and specific analyses based on the most sophisticated methods and using state-of-the-art analytical apparatuses. The extent of consulting, expertise, and research and development activities is not restricted to the areas listed above, but it may be further expanded according to specific needs of industrial practice.
I believe that this introduction will provide basic information about the orientation of the Faculty and various forms of study offered by its individual departments, and that it will help us find new partners for cooperation in solving research and professional problems.
prof. Ing. Karel Melzoch, CSc
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Numerous projects are interdisciplinary and take advantage of broad collaborations at the national and international scales. Research also takes advantage of the availability of sophisticated equipment and high-tech devices which employ emerging instrumentation principles.
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- Department of Biotechnology
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- Department of Saccharides and Cereals
- Department of Dairy, Fat and Cosmetic Science
- Department of Food Analysis and Nutrition
- Department of Food Preservation
- Department of Chemistry of Natural Compounds
- Accredited Testing Laboratories at FFBT
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Institute of Microbiology of the CAS
Advanced glycomimetics in neurodegeneration research
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | prof. Ing. Vladimír Křen, DrSc. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Salary |
Annotation
Alzheimer's disease (AD) represents a significant health and socioeconomic challenge. This project aims to develop innovative multi-targeted therapeutic approaches through the creation of dual/hybrid compounds. These compounds will combine optimized inhibitors of O-N-acetyl-?-D-glucosaminidase (OGA) and acetylcholinesterase (AChE) inhibitors using covalent conjugation via an optimized linker. Targeting both key biological receptors involved in AD progression offers the potential for more effective therapy. The study will include an evaluation of carbacyclic glycomimetics developed within the Czech-Austrian-Croatian collaboration, including testing their permeability across the blood-brain barrier (BBB) using static and dynamic models, increasing O-GlcNAcylation, and reducing Tau protein hyperphosphorylation. Advanced models of human AD, such as brain organoids, and in vivo efficacy testing in tauopathic mouse models will also be part of the research in collaboration with the Institute of Experimental Medicine of the Czech Academy of Sciences. The project combines cutting-edge research with practical applications and provides a unique opportunity to engage in a multidisciplinary team.
Central Nervous System diagnostics
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Microbiology ( in English language ) |
| Supervisor: | prof. Ing. Vladimir Havlíček, Dr. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Salary |
Annotation
Central nervous system infections cause acute changes in mental and physical brain functions, leading to chronic impairment of cognitive, memory, and motor abilities. These changes are related to alterations in brain neuronal signaling in susceptible brain regions. This Ph.D. topic aims to utilize matrix-assisted laser desorption/ionization-mass spectrometry imaging and highly multiplex immunohistochemistry-based mass spectrometry imaging to analyze and quantify molecular interactions between host and pathogen in cases of neuroaspergillosis caused by Aspergillus fumigatus and pneumococcal meningitis caused by Streptococcus pneumoniae. The Ph.D. project will determine the optimal transport mechanism for microbial secondary metabolites, such as toxins and quorum sensing molecules, across the blood-brain barrier. The study will analyze the impact of microbial metabolites on neurotransmitter signaling, specifically through the dopaminergic and glutamatergic pathways, in critically affected brain regions. The candidate biomarkers will successfully diagnose CNS infections in human cerebrospinal fluid samples based on the host-pathogen molecular fingerprint.
Defined chitosan conjugates for biomedical applications
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | doc. RNDr. Pavla Bojarová, Ph.D. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Scholarship |
Annotation
Chitin is the second most abundant polymer in nature. Partial deacetylation of chitin produces chitosan, a linear polymer composed mainly of glucosamine units (GlcN) and to a lesser extent of N-acetylglucosamine units (GlcNAc) linked ?(1?4) by glycosidic bonds. Chitosan is biocompatible and can therefore be advantageously used in a variety of biological and biomedical applications. For many biological applications, it is advantageous to work with shorter chains of chitosan, the so-called chitooligosaccharides (COS). COS have been intensively researched for decades in the fields of medicine, pharmaceuticals, textile industry, food industry and agriculture. Despite their tremendous potential use, poorly characterized heterogeneous mixtures are used in most studies due to the unavailability of well-defined COS. The thesis will focus on the preparation of authentic, pure and fully structurally characterized COS and chitosans and their analysis. Subsequently, the defined COS chains will be used as carriers for multivalent presentation of bioactive carbohydrates, and their bioactivities will be studied, especially in biological tests with lectins.
Degradation of bacterial signaling molecules by rational engineered Ntn-hydrolases as novel antibacterials
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biotechnology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Biotechnology ( in English language ) |
| Supervisor: | RNDr. Andrea Palyzová, Ph.D. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Scholarship |
Annotation
Growing antibiotic resistance will result globally in 10 million deaths annually by 2050. The targeted design of quorum-quenching (QQ) enzymes, negatively affecting the communication process in Gram-negative bacteria leading to biofilm formation, known as quorum sensing (QS) process, represents a clever strategy to effectively combat this threat. Construction and development of modified proteins from the Ntn-hydrolase group (e.g., ecPGA isolated from Escherichia coli) using genetic-molecular techniques (RF cloning, PCR-based methods, CRISPR/Cas technology) and biotechnological strategies (scale-up process, His tag purification) will lead to the develop of a unique QQ enzyme with properties for specific binding to a wider spectrum of HSL molecules (3-oxo-C12-HSL, C8-HSL, C6-HSL, C4-HSL). The designed modified enzymes will be used in in vitro interactions with selected HSL molecules, and their degradation activity will be monitored. Degradation efficiency will be tested in a biological model during biofilm development and maturation in significant HSL-dependent Gram-negative pathogens Pseudomonas aeruginosa, Klebsiella pneumoniae, and Aeromonas veronii. Biofilm morphology will be analyzed using electron microscopy, while the profile of signaling molecules synthesized in the bacterial population will be thoroughly examined using liquid chromatography with tandem mass spectrometry. The project will explore and enhance the biotechnological potential of these QQ enzymes to act as an alternative and complement to conventional antibiotic therapies and agents for medical and industrial applications in combating biofilm formation.
Enzymatic synthesis of biologically active chitooligomers
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | doc. RNDr. Pavla Bojarová, Ph.D. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Scholarship |
Annotation
Chitooligomers are ?-1-4-linked oligosaccharides composed of N-acetylglucosamine and glucosamine units and their biological activity depends mainly on their degree of polymerization, degree of acetylation, and acetylation pattern. Chitooligomers are known for their ability to induce an immune response in plants and could thus be used as natural crop protection agents against microbial pests. This PhD project will focus on the efficient enzymatic preparation of chitooligomers with different degrees of polymerization and acetylation, in particular chitooligomers with degrees of polymerization 6-10 and different degrees of acetylation are in demand and not yet available. Microbial natural and mutant glycosidases, particularly chitinases and ?-N-acetylhexosaminidases, and chitin deacetylases from bacterial and fungal sources will be used for this purpose. These enzymes will be expressed in microbial expression systems (E. coli, P. pastoris), characterized, and optimized for the synthesis of the desired chitooligomers. The biological activities of the prepared chitooligomers, especially concerning plant pest protection, will be tested in collaboration with Czech and foreign partners of the laboratory.
Flavonoid-derived metabolites as standards for metabolic studies
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Chemistry of Natural Compounds
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | doc. Ing. Kateřina Valentová, Ph.D. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Scholarship |
Annotation
Flavonoids are plant food components that are known to be intensively metabolized in the gut and to interact with and influence the composition of the gut microbiome. Flavonoids are also subject to rapid biotransformation in the II phase, whereby mainly corresponding sulfates and glucuronides are formed. The biotransformation of polyphenols by the gut microbiota also leads to cleavage of the C-ring and the formation of simple phenolic substances. The aim of this PhD project is the optimization of chemoenzymatic methods for the synthesis of a library of natural flavonoids (myricetin, luteolin, kaempferol, naringenin, hydroxyphenylacetic acid and hydroxyphenylpropionic acid) and their metabolites (sulfates, glucuronides and depsides). Natural and mutant enzymes from bacterial and fungal sources (arylsulfotransferases, glucuronidases, quercetinase) are used for this purpose. These enzymes are expressed in microbial expression systems (E. coli, P. pastoris), characterized and optimized for the synthesis of the desired metabolites. The compounds are then used as standards for metabolic studies carried out in collaboration with our partner laboratories.
Changes in the urinary microbiome and metabolome in response to the therapeutic intervention in female patients with overactive bladder symptoms
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biotechnology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Biotechnology ( in English language ) |
| Supervisor: | RNDr. Andrea Palyzová, Ph.D. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Scholarship |
Annotation
Research into the microbiome of the urinary tract has progressed dramatically over the last decade. Currently, more than 500 bacterial species have been identified in urine, indicating significant diversity in the microbiome of the niche. As our understanding of the urinary microbiome grows, there is evidence of differences in microbiome composition between healthy individuals and those with lower urinary tract diseases. Overactive bladder (OAB) is a chronic disease of the lower urinary tract dysfunction affecting up to 20% of the population, more often women than men, characterized by sudden urges to urinate with a negative impact on quality of life. The pathophysiology of OAB is multifactorial, including metabolic syndrome, affective disorders, sex hormone deficiency, and other factors. The standard treatment of OAB is mainly based on lifestyle modification, behavioural therapy, and pharmacotherapy with anticholinergics. This study aims to correlate characteristics of the urinary microbiome and metabolome with OAB symptom severity and then to evaluate changes in the urinary microbiome and metabolome in response to therapeutic intervention for OAB. The study will include 210 subjects, approximately 60 with OAB symptoms and 150 healthy controls. Inclusion criteria for the OAB group will be the presence of clinical symptoms, the OAB-V8 patient-reported outcome questionnaire and the nerve growth factor (NGF) levels. Standard molecular techniques of DNA isolation and purification, PCR amplification, and sequencing by massively parallel sequencing on the Illumina MiSeq platform, complemented by bioinformatic analysis, will be used to gain insights into the microbiome. In addition to the composition of the microbial community, the molecular mechanisms at the level of identification of specific low molecular weight metabolites will play a key role in the patient´s condition. We will use non-targeted metabolomics to obtain comprehensive molecular profiles and semi-quantitatively determine the levels of unique metabolites. This will provide new insights into the biochemical functions of the detected metabolites associated with OAB and identify potential biomarkers associated with the development of this disease.
Integrated Stress Response in survival or cell death.
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Biochemistry and Bioorganic Chemistry ( in English language ) |
| Supervisor: | Mgr. Leoš Valášek, Ph.D. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Scholarship |
Annotation
Eukaryotické buňky si vyvinuly několik mechanismů, jak se vyrovnat s různými stresory prostředí. Patří mezi ně komplexní signální dráha označovaná jako integrovaná stresová odpověď (ISR), která vede buňky buď k adaptaci nebo ke smrti. Zatímco jejími vnějšími spouštěči jsou kyslík, nedostatek živin nebo virové infekce, hlavním vnitřním stresorem je hromadění nesložených proteinů v lumen endoplazmatického retikula (ER). ISR může být také indukována aktivací onkogenů. Reakce je „integrovaná“, protože všechny stresové signály jsou přenášeny rodinou čtyř serinových/treoninových kináz a konvergují do jediné události, kterou je fosforylace ? podjednotky eukaryotického iniciačního faktoru translace 2 (eIF2?). Provedli jsme komplexní screening, abychom zjistili, jaké mRNA a lncRNA jsou překládány za normálních podmínek versus akutní nebo chronické (pro-apoptotické) ISR. Cílem navrhovaného projektu je vybrat klíčové mRNA z našeho robustního screenu a prozkoumat jednak molekulární mechanismus jejich specializované translace, jakož i úlohu jejich proteinových produktů za daných stresových podmínek.
Secondary metabolism of intracellular pathogens
| Study place: | Institute of Microbiology of the CAS |
| Guaranteeing Departments: |
Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS |
| Study Programme/Specialization: | Microbiology ( in English language ) |
| Supervisor: | prof. Ing. Vladimir Havlíček, Dr. |
| Expected Form of Study: | Full-time |
| Expected Method of Funding: | Salary |
Annotation
The PhD candidate will study, correlate and mine the metabolism of intracellular pathogens within host cells. The candidate can solve five pressing diagnostic problems of our time, and together with the Stanford University, can address the dynamic range of analytical chemistry in mapping and quantifying intracellular pathogens in the host. Through this collaboration, the applicant will be able to work with isogenic strains, i.e., will always have host systems with very similar metabolomics and identical host genetic makeup. All directions of intracellular research are of the highest societal importance and involve the endemic fungus Coccidioides immitis, a Pf4 bacteriophage in the Gram-negative bacterium Pseudomonas aeruginosa, the AfPmV-1 polyomavirus in the filamentous fungus Aspergillus fumigatus, and the betaproteobacterium Mycetohabitans endofungorum, an intracellular symbiont of the filamentous fungus Rhizopus microsporus. Also a contribution to a novel diagnostic for Mycobacterium tubercullosis in mammalian cells is expected. The applicant will use the portfolio of infection metallomics, a set of dependent mass spectrometry, separation, and computational techniques. ) ) [sablona] => stdClass Object ( [class] => stranka [html] => [css] => [js] => [autonomni] => 1 ) [api_suffix] => druh/I/jazyk/en/fakulta/22330/locale/en/ustav/004 )